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FLOW CYTOMETRY

Immunophenotypic analysis by multiparameter flow cytometry is a powerful tool used in determining the expression of cell surface and intracellular markers. Normal and abnormal cells can be identified, enumerated and characterized with respect to biomarker expression, cell lineage and maturation state. The sensitivity of the technology allows large amounts of data to be processed quickly and with rare event analysis. Our laboratory has experience validating novel antibodies for clinical trial flow analyses.

FLOW Markers

Flow cytometry utility
  • Biomarker analysis
  • Rare event analysis
  • Cell cycle analysis; DNA Ploidy and S Phase
  • Absolute lymphocyte subset counts
  • Cell viability
Flow cytometry markers

Lymphoid markers:
CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD23, CD38, CD45, CD56, CD57, sIgkappa, sIglambda,FMC7, T-gamma/delta

Lymphocyte profile and absolute counts:
 CD3, CD4, CD8, CD16, CD19, CD45, CD56

Myeloid Markers:
CD11b, CD13, CD14, CD16, CD33, CD34, CD45, CD117, HLADR

Natural Killercell markers:
CD16, CD56, CD57

Plasma cell markers:
CD38, CD45, CD56, CD138, cIg kappa, cIg lambda

Specialty markers:
 Ber-EP4 epithelial antigen
CD1, CD11c, CD11b, CD25, CD30, CD41, CD42b, CD52, CD61, CD64, CD103, CD117
EMA epithelial membrane antigen
Glycophoryin-A, CD71
HLA-B27
Nuclear TdT
Intracellular cytokeratin, myeloperoxiodase, CD3, CD22, CD79a, CD55, CD59, FLAER
bcl2
Ki-67
ZAP-70

Other markers available on request.
CYTOGENETICS & FISH

In addition to offering high quality chromosome analysis, the cytogenetics laboratory also specializes in fluorescence in situ hybridization (FISH). FISH, a molecular cytogenetic technique, enables the analysis of disease specific abnormalities as well as the chromosomal localization of specific DNA sequences. It is offered for the detection of cryptic rearrangements, microdeletion syndromes, aneuploidy and marker chromosome identification.
In addition to the listed probes provided in house, our laboratory services are available for validation and testing of novel FISH probes.

FISH Probes

Individual Probes

  • ALK 2p23 Anaplastic Large Cell Lymphoma
  • AML1/ETO t(8;21)
  • API2 / MALT1 t(11;18)
  • ATM deletion 11q22.3
  • BCL2, IGH/BCL2 t(14;18)
  • BCL1 IGH/CCND1 t(11;14)
  • BCL6 3q27 Rearrangement
  • BCR/ABL+9q34, t(9;22)
  • CBFB t(16;16), inv(16)
  • CEP 7 -7/7q31-
  • C MYC 8q24 Rearrangement
  • Deletion 13q14.3
  • Deletion 20q12
  • EGR1 -5/5q-
  • FIP1L1/PDGFRA 4q12 Rearrangement
  • IGH 14q32 Rearrangement
  • IGH/FGFR3 t(4;14)
  • IGH/MAF t(14;16)
  • IGH/MALT1 t(14;18)
  • IGH/MYC, CEP 8, t(8; 14)
  • MALT1 18q21
  • MLL 11q23
  • MYB deletion 6
  • p53 deletion 17p13
  • PML/RARA t(15; 17)
  • SRY/CEP X deletion Yp11.3
  • trisomy 3
  • trisomy 4
  • trisomy 5, 9 and 15
  • trisomy 8
  • trisomy 10
  • trisomy 12
  • TEL/AML1 t(12;21)





Tissue Based Probes

  • Oligodendroglioma 1p36/19q13- Glioma, Oligodendroglioma
  • UroVysion® : +3/+7/+17/deletion 9p21, Bladder
  • EGFR 7p12 Amplification, Lung, colon, breast
  • EWSR1 22q12 Rearrangement Ewing Sarcoma
  • HER2/neu PathVysion®, Breast
  • N-MYC 2p24.1, Neuroblastoma
  • PTEN/CEP10 deletion 10q23.3, Breast, endometrial, glioblastoma
  • SYT 18q11.2 Synovial Sarcoma

 
 
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